Special issue: Ribosome-inactivating proteins : commemorative issue in honor of Professor Fiorenzo Stirpe

The family of ribosome-inactivating proteins (RIPs) groups all enzymes (EC.3.2.2.22) with a so-called RIP domain which comprises N-glycosidase activity and enables these proteins to catalytically inactivate ribosomes.[...

Overexpression of Nictaba-like lectin genes from glycine max confers tolerance towards Pseudomonas syringae infection, aphid infestation and salt stress in transgenic Arabidopsis plants

Plants have evolved a sophisticated immune system that allows them to recognize invading pathogens by specialized receptors. Carbohydrate-binding proteins or lectins are part of this immune system and especially the lectins that reside in the nucleocytoplasmic compartment are known to be implicated in biotic and abiotic stress responses. The class of Nictaba-like lectins (NLL) groups all proteins with homology to the tobacco (Nicotiana tabacum) lectin, known as a stress-inducible lectin. Here we focus on two Nictaba homologs from soybean (Glycine max), referred to as GmNLL1 and GmNLL2. Confocal laser scanning microscopy of fusion constructs with the green fluorescent protein either transiently expressed in Nicotiana benthamiana leaves or stably transformed in tobacco BY-2 suspension cells revealed a nucleocytoplasmic localization for the GmNLLs under study. RT-qPCR analysis of the transcript levels for the Nictaba-like lectins in soybean demonstrated that the genes are expressed in several tissues throughout the development of the plant. Furthermore, it was shown that salt treatment, Phytophthora sojae infection and Aphis glycines infestation trigger the expression of particular NLL genes. Stress experiments with Arabidopsis lines overexpressing the NLLs from soybean yielded an enhanced tolerance of the plant towards bacterial infection (Pseudomonas syringae), insect infestation (Myzus persicae) and salinity. Our data showed a better performance of the transgenic lines compared to wild type plants, indicating that the NLLs from soybean are implicated in the stress response. These data can help to further elucidate the physiological importance of the Nictaba-like lectins from soybean, which can ultimately lead to the design of crop plants with a better tolerance to changing environmental conditions

Evolution of plant ribosome-inactivating proteins

This contribution presents an updated analysis of the evolution of ribosome-inactivating proteins (RIPs) in plants. All evidence suggests that an ancestor of modern seed plants developed the RIP domain at least 300 million years ago. This ancestral RIP domain gave rise to a direct lineage of type 1 RIPs (i.e. primary type 1 RIPs) still present today in many monocots and at least one dicot. In a later stage a plant succeeded in fusing the RIP domain to a duplicated ricin-B domain acquired from a bacterium. The resulting ancestral type 2 RIP gave rise to all modern type 2 RIPs and by domain deletion to different lines of ‘secondary’ type 1 RIPs and ricin-B type lectins. In a more recent past at least three other domain fusions took place in the family Poaceae whereby type AC1 (type 3), type AC2 and type AD chimeric forms were generated

Extensive evolution of cereal ribosome-inactivating proteins translates into unique structural features, activation mechanisms, and physiological roles

Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can depurinate rRNAs thereby inhibiting protein translation. Although these proteins have also been detected in bacteria, fungi, and even some insects, they are especially prevalent in the plant kingdom. This review focuses on the RIPs from cereals. Studies on the taxonomical distribution and evolution of plant RIPs suggest that cereal RIPs have evolved at an enhanced rate giving rise to a large and heterogeneous RIP gene family. Furthermore, several cereal RIP genes are characterized by a unique domain architecture and the lack of a signal peptide. This advanced evolution of cereal RIPs translates into distinct structures, activation mechanisms, and physiological roles. Several cereal RIPs are characterized by activation mechanisms that include the proteolytic removal of internal peptides from the N-glycosidase domain, a feature not documented for non-cereal RIPs. Besides their role in defense against pathogenic fungi or herbivorous insects, cereal RIPs are also involved in endogenous functions such as adaptation to abiotic stress, storage, induction of senescence, and reprogramming of the translational machinery. The unique properties of cereal RIPs are discussed in this review paper

ENMat international projects: FP7 NMP large collaborative project: 3D-LightTrans

Large scale manufacturing technology for high-performance lightweight 3D multifunctional composites The goal of the 3D-LightTrans project is to provide groundbreaking, highly flexible, efficient and adaptable low-cost technologies for the manufacturing of integral large scale 3D textile reinforced plastic composites, including innovative approaches for the individual processes and its integration in complete manufacturing chains, which will enable to shift them from its current position in cost intensive, small series niche markets, to broadly extended mass product applications, not only in transportation, but also in other key sectors, like health and leisure. To fulfil this goal, the 3DLightTrans manufacturing chains will be based on multimaterial semifinished fabrics, processed to deep draped prefixed multilayered and multifunctional 3D -textile preforms, which will be processed into composites by a thermoforming process. By integrating these new, innovative process steps with full automation in -nowadays mostly manually performed- complex handling operations, it will be possible to obtain a fully automated and highly adaptable manufacturing chain to achieve integral large scale 3D composites. 3D-LightTrans will open the way to a totally new concept for the design, manufacturing and application of composites for low-cost mass products in a wide range of sectors. The Consortium brings together multidisciplinary research teams involving European leading companies, including industrial stakeholders from machine tools and machine automation and several OEM active in the field of processing of flexible materials and composite manufacturing, as well as from the application sector, and extensive expertise from well known research specialists in the area of materials, production research and technical textiles in particular. Start date : 01/04/2011 Project duration : 4 years More information: Dr. Erich Kny Austrian Institute of Technology, [email protected] URL: http://www.3d-lighttrans.com

ENMat international projects: FP7 NMP coordination action: 2BFUNTEX

Boosting collaboration between research centres and industry to enhance rapid industrial uptake of innovative functional textile structures and textile-related materials in a mondial market 2BFUNTEX will exploit the untapped potential in functional textile structures and textile related materials. It will bring together all innovation actors in the field fostering a multidisciplinary approach between universities, research institutes, SMEs and sector associations. The 2BFUNTEX team will identify technological gaps and eliminate barriers resulting in a faster industrial uptake of added value functional materials with new functionalities and improved performance and resulting in creation of new business worldwide. Technological needs will be mapped, new joint international research disciplines will be identified and multidisciplinary lab teams will be created. International cooperation will be favoured to exploit the worldwide market expansion potential. Industry will be involved at all stages of the process. The inventory will enlarge the team of important textile universities and renowned materials research centres and will identify new collaborations. Synergy will be reinforced and created which will enable to identify and develop new functional materials. Training materials regarding functional materials for research and industrial purposes will be developed and implemented to allow a common language regarding functional textile structures and text ile related materials, and will increase the number of well-trained people in this field. Further, the 2BFUNTEX partners will organise and participate in conferences, workshops and brokerage events. Along with a website with an extensive database comprising all information gained throughout the project, collaboration will be boosted and rapid industrial uptake catalysed and enhanced. The project duration will be 4 years and the consortium includes 26 partners from 16 countries. Start date : 01/01/2012 More information: Ir. Els Van der Burght Department of Textiles/Ghent University [email protected] [email protected] URL: http://www.2bfuntex.e

Evaluation of the susceptibility of the pea aphid, Acyrthosiphon pisum, to a selection of novel biorational insecticides using an artificial diet

An improved technique was developed to assay the toxicity of insecticides against aphids using an artificial diet. The susceptibility of the pea aphid Acyrthosiphon pisum (Harris) (Hemiptera: Aphidoidea) was determined for a selection of novel biorational insecticides, each representing a novel mode of action. Flonicamid, a novel systemic insecticide with selective activity as feeding blocker against sucking insects, showed high toxicity against first-instar A. pisum nymphs with an LC50 of 20.4 mu g/ml after 24 h, and of 0.24 mu g/ml after 72 h. The toxicity was compared with another feeding blocker, pymetrozine, and the neonicotinoid, imidacloprid. In addition, four insect growth regulators were tested. The chitin synthesis inhibitor flufenoxuron, the juvenile hormone analogue pyriproxyfen, and the azadirachtin compound Neem Azal-T/S showed strong effects and reduced the aphid population by 50% after 3 days of treatment at a concentration of 7-9 mu g/ml. The ecdysone agonist tested, halofenozide, was less potent. In conclusion, the improved aphid feeding apparatus can be useful as a miniature screening device for insecticides against different aphid pests. The present study demonstrated rapid and strong toxicity of flonicamid, and other biorational insecticides towards A. pisum

Evaluating hospital performance based on excess cause-specific incidence

Formal evaluation of hospital performance in specific types of care is becoming an indispensable tool for quality assurance in the health care system. When the prime concern lies in reducing the risk of a cause-specific event, we propose to evaluate performance in terms of an average excess cumulative incidence, referring to the center's observed patient mix. Its intuitive interpretation helps give meaning to the evaluation results and facilitates the determination of important benchmarks for hospital performance. We apply it to the evaluation of cerebrovascular deaths after stroke in Swedish stroke centers, using data from Riksstroke, the Swedish stroke registry